clearly outline workflow, timeline, make pipetting tables, etc.
use STAR procedures (more information is helpful, once noted it is easily copied)
| Reagent / Resource | Source (links) | ID (cat nr) | Lot# | Working concentration | Q Score (5=best) | Remarks | | --- | --- | --- | --- | --- | --- | --- | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | | |
Extra information:
Antibodies: <Species>-anti-<Species ><Target>, clone, label, concentration stock, dilution-working concentration. (Species list: Gt, Rt, Rb, Ms, Hs, GP, Ll/VHH / …)
Plasmids/ primers: link to Benchling or plasmid map, elab, SNAPgene link
Chemicals: concentration (v/v; w/v; molarity NOT dilution), buffer
Fluorescent proteins/compounds/peptides: concentration, lot/batch, buffer, Dye (#fluorescent groups per mol/unit)
Cells: ATCC ref, (primary) isolate, pass nr, media (serum / selection additives)
Sample storage location: eLab inventory link.
Microscopy setting: scoring forms (Objective / light sources (power) / filters / sequential<>simultaneous …)
=> Checklists microscopy / MicroMeta App
Image Analysis: Software (version, open source: link-literature), used scripts (links-literature), supply custom written scripts (make sure dependencies are findable in the comments).